With the aim of establishing the basic knowledge and resources needed for applied genetics, we investigated the genome structure of red clover Trifolium pratense L. by a combination of cytological, genomic and genetic approaches. The deduced genome size was approximately 440 Mb, as estimated by measuring the nuclear DNA content by flow cytometry. Seven chromosomes could be distinguished by microscopic observation of DAPI stained prometaphase chromosomes and fluorescence in situ hybridization using 28S and 5S rDNA probes and bacterial artificial chromosome probes containing microsatellite markers with known positions on a genetic linkage map. The average GC content of the genomes of chloroplast, mitochondrion and nucleus were shown to be 33.8, 42.9 and 34.2

作者：Shusei, Sato；Sachiko, Isobe；Erika, Asamizu；Nobuko, Ohmido；Ryohei, Kataoka；Yasukazu, Nakamura；Takakazu, Kaneko；Nozomi, Sakurai；Kenji, Okumura；Irina, Klimenko；Shigemi, Sasamoto；Tsuyuko, Wada；Akiko, Watanabe；Mitsuyo, Kohara；Tsunakazu, Fujishiro；Satoshi, Tabata

来源：DNA research : an international journal for rapid publication of reports on genes and genomes 2005 年 12卷 5期