Aberrant DNA methylation is one of the major events in carcinogenesis. Promoter DNA methylation is also present in various non-neoplastic tissues including gastric epithelium as age-related phenomenon, suggesting that it occurs early in the process of tumorigenesis.We aimed to clarify the relationship of aberrant DNA methylation in non-neoplastic gastric epithelia with the risk of gastric cancer, Helicobactor pylori infection, and the degree of H. pylori-induced gastritis.89 patients enrolled in this study. The status of aberrant DNA methylation was compared in two groups of patients: 43 cases with gastric cancer (mean age 65.9 years [29-91], F:M = 0.30, intestinal type [n = 25], diffuse type [n = 18]) and 46 age- and sex-matched patients without gastric cancer (peptic ulcer diseases [n = 11], gastritis [n = 35]) as a control group. Genomic DNA was extracted directly from non-neoplastic epithelia of antral biopsies obtained by endoscopy. The promoter methylation status of the p14 and p21 genes was determined by methylation-specific-polymerase chain reaction (MSP). The promoter methylation status of the p16 gene was quantified by digital densitographic analysis following MSP. The degree of gastritis in the antrum was assessed according to the updated Sydney system. The PG I/II ratio was calculated based on the data of serum PG I and PG II levels measured by radioimmunoassay.In all 89 subjects, CpG island methylation was found in 25.8

作者：Tomomitsu, Tahara；Tomiyasu, Arisawa；Tomoyuki, Shibata；Fang Yu, Wang；Masakatsu, Nakamura；Mikijyu, Sakata；Mitsuo, Nagasaka；Tamaki, Takagi；Yoshio, Kamiya；Hiroshi, Fujita；Masahiko, Nakamura；Shin, Hasegawa；Masami, Iwata；Kazuya, Takahama；Makoto, Watanabe；Ichiro, Hirata；Hiroshi, Nakano

来源：Digestion 2007 年 75卷 1期