To identify and validate potential biomarkers of colorectal adenocarcinoma using a proteomic approach.Multidimensional liquid chromatography⁄mass spectrometry was used to analyze biological samples labelled with isobaric mass tags for relative and absolute quantitation to identify differentially expressed proteins in human colorectal adenocarcinoma and paired normal mucosa for the discovery of cancerous biomarkers. Cancerous and noncancerous samples were compared using online and offline separation. Protein identification was performed using mass spectrometry. The downregulation of gelsolin protein in colorectal adenocarcinoma samples was confirmed by Western blot analysis and validated using immunohistochemistry.A total of 802 nonredundant proteins were identified in colorectal adenocarcinoma samples, 82 of which fell outside the expression range of 0.8 to 1.2, and were considered to be potential cancer-specific proteins. Immunohistochemistry revealed a complete absence of gelsolin expression in 86.89

作者：Nai-Jun, Fan；Chun-Fang, Gao；Chang-Song, Wang；Jing-Jing, Lv；Guang, Zhao；Xin-Hua, Sheng；Xiu-Li, Wang；Dong-Hui, Li；Qing-Yin, Liu；Jian, Yin

来源：Canadian journal of gastroenterology = Journal canadien de gastroenterologie 2012 年 26卷 1期