Multicolour single molecule fluorescence imaging enables the study of multiple proteins in the membranes of living cells. We describe the use of a supercontinuum laser as the excitation source, show its comparability with multiplexed single-wavelength lasers and demonstrate that it can be used to study membrane proteins such as the ErbB receptor family. We discuss the benefits of white-light sources for single molecule fluorescence, in particular their ease of use and the freedom to use the most appropriate dye without being constrained by available laser wavelengths.

作者：Stephen E D, Webb；Laura, Zanetti-Domingues；Benjamin C, Coles；Daniel J, Rolfe；Richard J, Wareham；Marisa L, Martin-Fernandez

来源：Biomedical optics express 2012 年 3卷 3期