A simple, sensitive, and rapid HPLC-MS/MS method was developed and validated for quantitative estimation of memantine in human plasma. Chromatography was performed on Zorbax SB-C(18) (4.6 × 75 mm, 3.5 μm) column. Memantine (ME) and internal standard Memantine-d6(MED6) were extracted by using liquid-liquid extraction and analyzed by LC-ESI-MS/MS using multiple-reaction monitoring (MRM) mode. The assay exhibited a linear dynamic range of 50.00-50000.00 pg/ml for ME in human plasma. This method demonstrated an intra- and interday precision within the range of 2.1-3.7 and 1.4-7.8

作者：Ravi Kumar, Konda；B R, Challa；Babu Rao, Chandu；Kothapalli B, Chandrasekhar

来源：Journal of analytical methods in chemistry 2012 年 2012卷