Allantoin in human plasma is a specific biomarker of oxidative stress. We describe a sensitive method to measure plasma allantoin using isocratic liquid chromatography and mass spectrometry (LC-MS/MS).Direct injection of deproteinized plasma into the LC-MS/MS system was performed. The method was technically evaluated. Results on 200 healthy and 35 Type 2 diabetic Chinese subjects were compared.Dose-response of allantoin was linear to at least 21 pmol (20 μmol/l in plasma); LOD was 0.16 pmol; recovery 99.2-100.2
作者：Wai-Yuen, Chung；Iris F F, Benzie
来源：Clinica chimica acta; international journal of clinical chemistry 2013 年 424卷
