Human papillomavirus (HPV) is a major etiologic factor for oropharyngeal squamous cell carcinoma (OPSCC). However, little is known about HPV-related OPSCC in Japan. During the study, formalin-fixed, paraffin-embedded OPSCC specimens from Japanese patients were analyzed for HPV DNA by the polymerase chain reaction (PCR) and for the surrogate marker p16 by immuno-histochemistry. For HPV DNA-positive, p16-negative specimens, the methylation status of the p16 gene promoter was examined by methylation-specific PCR. Overall survival was calculated in relation to HPV DNA and p16 status and was subjected to multivariate analysis. OPSCC cell lines were examined for sensitivity to radiation or cisplatin in vitro. The study results showed that tumor specimens from 40 (38

作者：Hisato, Kawakami；Isamu, Okamoto；Kyoichi, Terao；Kazuko, Sakai；Minoru, Suzuki；Shinya, Ueda；Kaoru, Tanaka；Kiyoko, Kuwata；Yume, Morita；Koji, Ono；Kazuto, Nishio；Yasumasa, Nishimura；Katsumi, Doi；Kazuhiko, Nakagawa

来源：Cancer medicine 2013 年 2卷 6期