Interleukin-18 (IL-18) is a pro inflammatory cytokine which plays a key role in the acute and chronic inflammatory phases of Rheumatoid Arthritis (RA). The Single Nucleotide Polymorphisms (SNPs) of IL-18 gene promoter region at positions -137 and -607, are postulated to be associated with RA. To test this, this study aimed to identify the association between these SNPs of the IL-18 gene promoter region of RA in south Indian patients.This study was carried on 190 subjects among which 90 were RA patients and 100 were age and sex matched controls. Genomic DNA was extracted by Salting out method. IL 18 gene promotor region SNPs, IL 18 - 607 and IL 18 -137 were amplified by using sequence specific primers. The amplified products of different samples were separated by using a 1.5

作者：Varun Chaithanya, Gurram；Sunil Kumar, Polipalli；Vijay Kumar, Karra；Madhavi, Puppala；Sanjeev Kumar, Pandey；Seema, Kapoor；Ashok, Kumar；Sudhakar, Godi

来源：Journal of clinical and diagnostic research : JCDR 2014 年 8卷 6期