Simultaneous monitoring of CMV and BKV by quantitative PCR in renal transplant recipients.-临床诊疗知识库

Simultaneous monitoring of CMV and BKV by quantitative PCR in renal transplant recipients.

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作者：

Cun-Zao, Wu；Xiao-Qian, Chen；Zhang-Yang, Wang；Xiao-Dong, Pan；Yong-Heng, Bai；Yi-Rong, Yang；Shao-Ling, Zheng；Peng, Xia

来源：

Journal of virological methods 2014 年 210卷

标签：

Cytomegalovirus Polyomavirus Q-PCR Renal transplantation

Polyomavirus (BKV) and cytomegalovirus (CMV) are associated with renal graft failure. The aim was to establish a quantitative PCR method (Q-PCR) to detect BKV and CMV simultaneously. The conserved sequences of BKV and CMV were amplified and cloned into the plasmids as standards. The sensitivity, specificity and the precision of the assay were evaluated. Q-PCR was used to detect BKV and CMV DNA simultaneously in 480 blood samples of renal transplantation recipients. The sensitivity of the Q-PCR assay to detect BKV or CMV DNA reached 5×10(3)copies/mL. The use of control DNA verified that the assay could specifically detect the target DNA. The precision of the assay to quantify target DNA copies was acceptable (ICV 3.44

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