Prevalence and Molecular Diagnosis of Babesia ovis and Theileria ovis in Lohi Sheep at Livestock Experiment Station (LES), Bahadurnagar, Okara, Pakistan.-临床诊疗知识库

Prevalence and Molecular Diagnosis of Babesia ovis and Theileria ovis in Lohi Sheep at Livestock Experiment Station (LES), Bahadurnagar, Okara, Pakistan.

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作者：

Waseem, Shahzad；Haider, Noor；Mansur-Ud-Din, Ahmad；Rashid, Munir；Muhammad, Sharif Saghar；Muhammad, Hassan Mushtaq；Nisar, Ahmad；Ghulam, Akbar；Fayyaz, Mehmood

来源：

Iranian journal of parasitology 2013 年 8卷 4期

标签：

Babesia ovis PCR Pakistan Sheep Theileria ovis

Babesia ovis and Theileria ovis are among the important and main etiological agents causing ovine babesiosis and ovine theileriosis, causing severe economic losses among sheep and goats. The aim of the present study was to determine the prevalence and molecular diagnosis of B. ovis and T. ovis in Lohi sheep at Livestock Experiment Station Bahadurnagar, Okara, Pakistan.The prevalence of B. ovis and T. ovis was investigated in 200 Lohi sheep of mixed age and sex by PCR during 2011. The assay was employed using primers Bbo-F & Bbo-R, specific for a 549-bp fragment in B. ovis genomic DNA and primers TSsr 170F & TSsr 670R, specific for a 520-bp fragment in T. ovis genomic DNA. The animals were also screened for both haemoparasites through stained thin blood smears.Thirty two (16

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