Quinolones have long been used as the first-line treatment for Campylobacter infections. However, an increased resistance to quinolones has raised public health concerns. The development of new quinolone-based antibiotics with high activity is critical for effective, as DNA gyrase, the target of quinolones, is an essential enzyme for bacterial growth in several mechanisms. The evaluation of antibiotic activity against Campylobacter jejuni largely relies on drug susceptibility tests, which require at least 2 days to produce results. Thus, an in vitro method for studying the activity of quinolones against the C. jejuni DNA gyrase is preferred. To identify potent quinolones, we investigated the interaction of C. jejuni DNA gyrase with a number of quinolones using recombinant subunits. The combination of purified subunits exhibited DNA supercoiling activity in an ATP dependent manner. Drug concentrations that inhibit DNA supercoiling by 50

作者：Ruchirada, Changkwanyeun；Masaru, Usui；Siriporn, Kongsoi；Kazumasa, Yokoyama；Hyun, Kim；Orasa, Suthienkul；Kanjana, Changkaew；Chie, Nakajima；Yutaka, Tamura；Yasuhiko, Suzuki

来源：Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2015 年 21卷 8期