1,3-Butadiene (BD) is a ubiquitous environmental pollutant found in tobacco smoke. In vivo, BD is mainly metabolized to form monohydroxybutenylmercapturic acid (MHBMA) and N-acetyl-S-(3, 4-dihydroxybutyl) cysteine (DHBMA). The accurate quantification of MHBMA and DHBMA in urine may provide important insights into the actual internal exposure of the general population to BD. 8-Hydroxy-2-deoxyguanosine (8-OHdG) is the biomarker of oxidative damage. In this study, a column-switching LC-MS/MS method was developed and validated for the simultaneous quantification of MHBMA, DHBMA derived from BD exposure and 8-OHdG in human urine. Urine samples were loaded on a LiChrospher(®) RP-8 ADS (25μm) 25×4mm RAM column for the extraction and clean-up of analytes. The separation was achieved using a SUPELCO(®) LC-18-DB column (75mm×3.0mm, 3μm). The analytes were ionized in negative electrospray ionization mode and analyzed in multiple reaction monitoring mode. Under optimum conditions, recoveries ranged from 78.9

作者：Xiaotao, Zhang；Hongwei, Hou；Huan, Chen；Yong, Liu；An, Wang；Qingyuan, Hu

来源：Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2015 年 1002卷