Serological diagnostic tests for canine and human leishmaniasis present problems related with their sensitivity and/or specificity. Recently, an immunoproteomic approach performed with Leishmania infantum proteins identified new parasite antigens. In the present study, the diagnostic properties of two of these proteins, cytochrome c oxidase and IgE-dependent histamine-releasing factor, were evaluated for the serodiagnosis of canine visceral (CVL) and human tegumentary (HTL) leishmaniasis. For the CVL diagnosis, sera samples from non-infected dogs living in an endemic or non-endemic area of leishmaniasis, sera from asymptomatic or symptomatic visceral leishmaniasis (VL) dogs, from Leish-Tec(®)-vaccinated dogs, and sera from animals experimentally infected by Trypanosoma cruzi or Ehrlichia canis were used. For the HTL diagnosis, sera from non-infected subjects living in an endemic area of leishmaniasis, sera from active cutaneous or mucosal leishmaniasis patients, as well as those from T. cruzi-infected patients were employed. ELISA assays using the recombinant proteins showed both sensitivity and specificity values of 100

作者：Eduardo Antonio Ferraz, Coelho；Lourena Emanuele, Costa；Daniela Pagliara, Lage；Vívian Tamietti, Martins；Esther, Garde；Nathália Cristina, de Jesus Pereira；Eliane Gon?alves Paiva, Lopes；Luiz Felipe Nunes Menezes, Borges；Mariana Costa, Duarte；Daniel, Menezes-Souza；Danielle Ferreira, de Magalh?es-Soares；Miguel Angel, Chávez-Fumagalli；Manuel, Soto；Carlos Alberto Pereira, Tavares

来源：Veterinary parasitology 2016 年 215卷