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Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The maximum FRET efficiency for the developed nano-biosensor was observed at 60

作者:Taha Roodbar, Shojaei;Mohamad Amran Mohd, Salleh;Kamaruzaman, Sijam;Raha Abdul, Rahim;Afshin, Mohsenifar;Reza, Safarnejad;Meisam, Tabatabaei

来源:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2016 年 169卷

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作者:
Taha Roodbar, Shojaei;Mohamad Amran Mohd, Salleh;Kamaruzaman, Sijam;Raha Abdul, Rahim;Afshin, Mohsenifar;Reza, Safarnejad;Meisam, Tabatabaei
来源:
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2016 年 169卷
标签:
Bioconjugation Cadmium-telluride quantum dots Fluorometric immunoassay Gold nanoparticles Nanobiosensor Quenching
Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The maximum FRET efficiency for the developed nano-biosensor was observed at 60