Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.
作者:Kazuo, Imai;Norihito, Tarumoto;Kiyoko, Amo;Makoto, Takahashi;Naoya, Sakamoto;Atsushi, Kosaka;Yasuyuki, Kato;Kei, Mikita;Jun, Sakai;Takashi, Murakami;Yutaka, Suzuki;Shigefumi, Maesaki;Takuya, Maeda
来源:Parasitology international 2017 年