An accurate and simple-to-perform new version of a competitive ELISA (cELISA) kit that became commercially available in 2015 for testing of cattle for antibody to Anaplasma marginale was validated for detection of Anaplasma ovis antibody in domestic sheep. True positives and negatives were identified using nested PCR (nPCR) as the gold standard. Negative bovine control sera supplied with the kit were used to calculate
作者:Kathleen L, Mason;Michael V, Gonzalez;Chungwon, Chung;Michelle R, Mousel;Stephen N, White;Joshua B, Taylor;Glen A, Scoles
来源:Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2017 年