Collagen type 4 alpha 1 (COL4A1) and collagen type 13 alpha 1 (COL13A1) produced by urothelial cancer cells support the vital oncogenic property of tumor invasion. We investigated the diagnostic and prognostic capability of COL4A1 and COL13A1 in voided urine and compared the observed values with those of CYFRA21-1, NMP-22, and voided urine cytology in bladder cancer (BCa). We collected voided urine samples from 154 patients newly diagnosed with BCa, before surgery and from 61 control subjects. Protein levels of COL4A1, COL13A1, CYFRA21-1, and NMP-22 in urine supernatants were measured using enzyme-linked immunosorbent assays. The diagnostic performance and optimal cutoff values were determined by receiver operating characteristic analysis. Urine levels of COL4A1, COL13A1, the combined values of COL4A1 and COL13A1 (COL4A1+COL13A1), and CYFRA21-1 were significantly elevated in urine from patients with BCa compared to the controls. Among these biomarkers, the optimal cutoff value of COL4A1+COL13A1 at 1.33 ng/mL resulted in 57.4

作者：Makito, Miyake；Yosuke, Morizawa；Shunta, Hori；Yoshihiro, Tatsumi；Sayuri, Onishi；Takuya, Owari；Kota, Iida；Kenta, Onishi；Daisuke, Goto；Yasushi, Nakai；Satoshi, Anai；Yoshitomo, Chihara；Kazumasa, Torimoto；Katsuya, Aoki；Nobumichi, Tanaka；Keiji, Shimada；Noboru, Konishi；Kiyohide, Fujimoto

来源：Cancer science 2017 年