Background and Objective:Cytokine-induced killer (CIK) cells and autologous dendritic cells-CIK (DC-CIK) cells co-cultured with autologous dendritic cells (DCs) and CIK cells are commonly used for immunotherapy recently.We compared the anti-tumor immune response of CIK cells, autologous DC-CIK cells, and semi-alloganeic DC-CIK cells to explore a more effective anti-tumor adoptive immunotherapy approach. Methods:Peripheral monocytes were isolated from patients with renal carcinoma, lung cancer, or maxillary squamous cell carcinoma and their healthy adult children. Isolated cells were cultured and induced as DCs and CIK cells in vitro. CIK cells from patients were co-cultured with autologous DCs and DCs from their children respectively, generating DC-CIK cells and semi-allogeneic DC-CIK cells. The anti-tumor activities of autologous CIK cells, autologous DC-CIK cells, and semi-allogeneic DC-CIK cells were measured by LDH assay. Intracellular staining was used to test the secretion of cytokines. Flow cytometry

作者：王其京；王慧；潘科；Yong-Qiang Li；Li-Xi Huang；Shi-Ping Chen；Jia He；Miao-La Ke；Jing-Jing Zhao；Jian-Jun Li；Jian-Cong Sun；Xiao-Ting Liang；Hai-Qing Ma；Yi-Bing Chen；Jian-Chuan Xia

来源：癌症 2010 年 29卷 7期