BACKGROUND:Human-mammal chimeric liver chimera has been a vital significance for the proliferation and differentiation of bone marrow mesenchymal stem cel s.<br> OBJECTIVE:To establish an animal model of human-rhesus chimeric liver using adult bone marrow mesenchymal stem cel s.<br> METHODS:Adult bone marrow mesenchymal stem cel s were isolated, purified and cultured for the sixth generation. The number of bone marrow mesenchymal stem cel s was no less than 5×108. Bone marrow mesenchymal stem cel s labeled with green fluorescent protein were transplanted into the liver of the embryo rhesus with pregnancy of 10 weeks under guided by type-B ultrasound. At the 1st and 3rd months of birth, the liver tissue of the infant rhesus was taken for biopsy. After routine pathological section, histological specimens were observed under fluorescence microscope to confirm if there were adult bone marrow mesenchymal stem cel s positive for green fluorescent protein and their distribution, and detected by immunohistochemical
作者:何保丽;马丽花;陈丽玲;刘汝文;杨仁华
来源:中国组织工程研究 2013 年 45期