N-acetylglucosaminyltransferase-V (GnT-V) is an enzyme that catalyzes beta1-6 branching of N-acetylglucosamine on asparagines (N)-linked oligosaccharides of cell proteins. We examined the implication of GnT-V and beta1-6 branching N-linked oligosaccharide expression in human testicular germ cells during malignant transformation and cancer progression. We analyzed immuhistochemically orchiectomy specimens of 130 patients with testicular germ cell tumors (TGCT) using anti-GnT-V monoclonal antibody, and compared GnT-V expression with clinicopathological features. N-linked oligosaccharide structural analysis was also performed to confirm the oligosaccharide profile produced by GnT-V. GnT-V was positive in all normal testis samples. This positive incidence declined in TGCT according to clinical stage; 16/71 (22.5

作者：Atsushi, Kyan；Noritaka, Kamimura；Shigeru, Hagisawa；Shingo, Hatakeyama；Takuya, Koie；Takahiro, Yoneyama；Yoichi, Arai；Hiroaki, Nakagawa；Shin-Ichiro, Nishimura；Eiji, Miyoshi；Yasuhiro, Hashimoto；Chikara, Ohyama

来源：International journal of oncology 2008 年 32卷 1期