We report the electrochemical detection of the redox active cardiac biomarker myoglobin (Mb) using aptamer-functionalized black phosphorus nanostructured electrodes by measuring direct electron transfer. The as-synthesized few-layer black phosphorus nanosheets have been functionalized with poly-l-lysine (PLL) to facilitate binding with generated anti-Mb DNA aptamers on nanostructured electrodes. This aptasensor platform has a record-low detection limit (∼0.524 pg mL(-1)) and sensitivity (36 μA pg(-1) mL cm(-2)) toward Mb with a dynamic response range from 1 pg mL(-1) to 16 μg mL(-1) for Mb in serum samples. This strategy opens up avenues to bedside technologies for multiplexed diagnosis of cardiovascular diseases in complex human samples.

作者：Vinod, Kumar；Jack R, Brent；Munish, Shorie；Harmanjit, Kaur；Gaganpreet, Chadha；Andrew G, Thomas；Edward A, Lewis；Aidan P, Rooney；Lan, Nguyen；Xiang Li, Zhong；M Grace, Burke；Sarah J, Haigh；Alex, Walton；Paul D, McNaughter；Aleksander A, Tedstone；Nicky, Savjani；Christopher A, Muryn；Paul, O'Brien；Ashok K, Ganguli；David J, Lewis；Priyanka, Sabherwal

来源：ACS applied materials & interfaces 2016 年 8卷 35期