Purpose:To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells(RCECs)in vitro and explore its pharmacological mechanism.Methods: The fresh rabbit cornea was cultured to get the primary RCECs,and RCECs of passage 2 were used for the research.The cells were divided into experimental groups,the cells in which were incubated with different concentrations(18.18,27.27,36.36,45.45,54.55 μg/ml)of dielofenac sodium,and control group.The effect of diclofenac sodium on the proliferation of cells was measured by methyl thiazolyl thiazolium(MTT)assay 24,48 and 72 h after incubation.While the RCECs were divided into experimental groups,the cells in which were incubated with 9 and 12.5μg/ml diclofenac sodium,and control group.The cell cycle and apoptotic rate were observed by flow cytometer.Results:MIT assay showed that diclofenac sodium had obvious inhibitory effect on RCECs,and the inhibition rate was increasing along with the increase of the concentration of diclofenac sodium and

来源：眼科学报 2010 年 25卷 2期