OBJECTIVETo explore the distribution frequencies of vitamin D receptor (VDR) gene polymorphisms in Chinese and the relationship between VDR genotypes and bone mineral density (BMD) or serum osteocalcin level in Chinese women.METHODSPolymorphisms of VDR gene were analyzed by polymerase chain reaction (PCR) to amplify the DNA sequence and three restriction enzymes (namely BsmI, ApaI and TaqI) to digest the PCR products in 223 subjects. Some of the VDR genotypes were also confirmed by Southern hybridization analysis. BMD was measured at the spine and proximal femur by dual-energy X-ray absorptiometry (DEXA). Serum osteocalcin concentrations were determined by radioimmunoassay.RESULTSChinese had high frequencies of "b,a,T" alleles, 95

作者：Zhao Jinxiu；Zhou Xueying；Meng Xunwu；Liu Guoyang；Xing Xiaoping；Liu Huaicheng；Xu Ling

来源：CHINESE MEDICAL JOURNAL 1997 年 110卷 5期