Background::The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p.Methods::We measured the expression of miR-296-5p in human OS cell lines and tissues. The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation, migration, and invasion of human OS lines was examined. The Student’s
t test was used for statistical analysis.
Results::We found that microRNA (miR)-296-5p was significantly downregulated in OS cell lines and tissues (control
vs. OS, 1.802 ± 0.313
vs. 0.618 ± 0.235,
t= 6.402,
P < 0.01). Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OA cells.
SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay. Overexpression of
SND1 abrogated the effects i
作者:Huang Ya-Zeng;Zhang Jun;Shen Jian-Jian;Zhao Ting-Xiao;Xu You-Jia
来源:中华医学杂志英文版 2021 年 134卷 5期