Objective　To deter mice the role of glucose in the control of insulin secretion and the possible mechanisms with which glucose fails to exert its effects on adenosine triphosphate-sensitive K＋ channels. Methods NMRI mice islets were used to investigate the amount of insulin secretion and the changes of energy state in islets when the activity of adenosine triphosphate-sensitive K＋channels was eliminated by diazoxide. Isolated islets were cutured in PRMI1640 medium for 18 hours, and were divided into 6 batches randomly, with 10 islets per batch （n＝20）. These were placed in 1.0 ml Krebs medium containing 250 μ mol/L diazoxide, 30 mmol/LK＋and variou glucose concentrations, respectively, and incubated in 37℃ water for 60 minutes. A portion of the supernatant was withdrawn at the end of the incubation for insulin assay, the islets were treated with trichloracetic acid and diethyl ether for the measurements of ATP, ADP, GTP, and UTP. Results　Under our experimental conditions, insulin secretion increased f

作者：肖常青；施焕中

来源：中华内科杂志 1998 年 37卷 zk期