A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantitatively analyzing T-2 toxin and its major metabolites (HT-2 toxin and T-2 triol) in swine biological samples. For all matrices, liquid-liquid extraction (ethyl acetate or acetonitrile) and Varian Bond-Elut MycoSep cartridges for solid phase extraction were used for sample preparation. The analytes were separated via a Zorbax XDB-C18 column and were detected using LC-MS/MS with an electrospray ionization interface in positive ion mode. The resulting calibration curves offered satisfactory linearity (r(2)>0.992) within the test range. The limits of quantification for T-2 toxin, HT-2 toxin, and T-2 triol were 1ng/mL (μg/kg), 2ng/mL (μg/kg), and 5ng/mL (μg/kg), respectively. The recovery rates in different matrices ranged from 74.3

作者：Yongxue, Sun；Guijun, Zhang；Haiyan, Zhao；Jianlong, Zheng；Fangyuan, Hu；Binghu, Fang

来源：Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2014 年 958卷